Recombinant Human Adiponectin (gAcrp30, ADIPOQ)
Purified No Carrier Protein
(Adipolean [human],ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28, Adipocyte Complement-Related Protein of 30)
| Prod. No.: | A222 |
| Source | NSO Cells |
| Pkg. Size: | 1 mg, 25 µg |
| Storage: | 2°C to 8°C Detailed storage instructions below. |
Description
Background:
gAdiponectin (gAcrp30) is an adipocyte complement-related protein and is a member of the family of defense collagens (1). gAdiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. The signaling receptors for adiponectin and gAcrp30 have recently been identified and named AdipoR1 and AdipoR2 (2). AdipoR1 is produced primarily in muscle while AdipoR2 is predominantly expressed in the liver. gAcrp30 is an anti-diabetic and anti-antherogenic hormone that plays important roles in the regulation of lipid and glucose metabolism (3).
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human gAcrp30 is Mr 16.7 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 20 kDa.
State of Matter
Solution
Formulation
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This protein can be stored under sterile conditions at 2° to 8°C for four months without detectable change of its oligomeric state.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Amino Acid Sequence
ayv yrsafsvgle tyvtipnmpi rftkifynqq nhydgstgkf hcnipglyyf ayhitvymkd vkvslfkkdk amlftydqyq ennvdqasgs vllhlevgdq vwlqvygege rnglyadndn dstftgflly hdtnhhhhhh
Country of Origin
USA
References
1. Scherer, P. E. et al. (1995) J. Biol. Chem. 270:26746
2. Yamauchi, T. et al. (2003) Nature 423:762
3. Hu, E. et al. (1996) J. Biol. Chem. 271:10697
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
