Bcl-2 (NT) Blocking Peptide
Blocking Peptide
| Prod. No.: | B472 |
| Conc.: | 0.2 mg/ml |
| Pkg. Size: | 50 µg |
| Storage: | -20°C Detailed storage instructions below. |
Description
Background:
Bcl-2 peptide is applicable as a control peptide for blocking antibody binding in Western blotting.
Amino Acid Location
15 amino acids near the amino-terminus of human Bcl-2.
Formulation
This peptide is formulated in PBS pH 7.2 (0.01 M Sodium Phosphate, 0.13 M NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage and Stability
Store this peptide in working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), N, WB
Neutralization: The peptide may be used to neutralize the binding activity of Anti-Human Bcl-2 (NT) (
Leinco Prod. No.: B446) by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51.
2. Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5α restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
3. Hatziioannou T, Perez-Caballero D, Yang A, et al. Retrovirus resistance factors REF1 and Lv1 are species-specific variants of TRIM5α. Proc. Nat’l. Acad. Sci. USA 2004; 101:10774-9
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
