Recombinant Mouse B7-Homology 1 (B7-H1)
Purified No Carrier Protein
(B7-H, MGC142294, MGC142296, PD-L1, PDCD1L1, PDCD1LG1, PDL1)
| Prod. No.: | B526 |
| Source | NSO Cells |
| Pkg. Size: | 1 mg, 100 µg |
| Storage: | -20°C to -70°C Detailed storage instructions below. |
Description
Background:
B7-H1, also called programmed death ligand (PD-L1) is a protein in the B7 family of immune proteins. PD-L1 binds to its receptor, PD-1, found on activated T cells, B cells, and myeloid cells, to modulate activation or inhibition. Engagement of PD-L1 with PD-1 on T cells delivers a signal that inhibits TCR-mediated activation of IL-2 production and T cell proliferation. The mechanism involves inhibition of ZAP-70 phosphorylation and its association with CD247 (1). PD-L1 signaling attenuates PKC-8 activation loop phosphorylation (resulting from TCR signaling), necessary for the activation of transcription factors NF-κB and AP-1 and for the production of IL-2. Upon IFN-γ stimulation, PD-L1 is expressed on T cells, NK cells, macrophages, myeloid DCs, B cells, epithelial cells, and vascular endothelial cells (2). Type I interferons can also upregulate PD-L1 on murine hepatocytes, monocytes, DCs, and tumor cells (3). It appears that upregulation of PD-L1 is a mechanism that cancers can employ to evade the host immune system as patients with higher expression levels of PD-L1 had a significantly poorer prognosis (4).
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse B7-H1 is Mr 51.3 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 75-85 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse B7-H1 was determined by its ability to inhibit anti-CD3 induced proliferation of 72 hour PHA T cell blast. The expected ED50 for this effect is typically 2.5 - 10 μg/mL.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
ft itapkdlyvv eygsnvtmec rfpvereldl lalvvyweke deqviqfvag eedlkpqhsn frgraslpkd qllkgnaalq itdvklqdag vycciisygg adykritlkv napyrkinqr isvdpatseh elicqaegyp eaeviwtnsd hqpvsgkrsv ttsrtegmll nvtsslrvna tandvfyctf wrsqpgqnht aeliipelpa thppqnrtie grmdpkscdk thtcppcpap ellggpsvfl fppkpkdtlm isrtpevtcv vvdvshedpe vkfnwyvdgv evhnaktkpr eeqynstyrv vsvltvlhqd wlngkeykck vsnkalpapi ektiskakgq prepqvytlp psrdeltknq vsltclvkgf ypsdiavewe sngqpennyk ttppvldsdg sfflyskltv dksrwqqgnv fscsvmheal hnhytqksls lspgk
Country of Origin
USA
References
1. Sheppard, K. A. et al. (2004) FEBS Letters 574:37
2. Flies, D. B. et al. (2007) J. Immunol. 30:251
3. Yamazaki, T. et al. (2002) J. Immunol. 169:5538
4. Thompson, R. H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:17174
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
