Recombinant Human B7-1
Purified No Carrier Protein
(CD80, LAB7, CD28LG, CD28LG1)
| Prod. No.: | B532 |
| Source | NSO Cells |
| Pkg. Size: | 1 mg, 100 µg |
| Storage: | -20°C to -70°C Detailed storage instructions below. |
Description
Background:
The B-lymphocyte activation antigen B7-1, also known as CD80, is a member of cell surface immunoglobulin superfamily. It is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as a dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and CTLA-4 expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production and the generation of CTL (1). Binding of this protein with CTLA-4 is involved in down-regulation of immune response. Contacts between B and T helper cells mediated by B7-1 provide negative signal for the proliferation and IgG secretion of normal B cells and B cell lymphomas. In addition, B7-1 also plays a role in induction of innate immune responses by activating NF-κB signaling pathway in macrophages. B7-1 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas (3).
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human B7-1 is Mr 51.3 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 70-90 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human B7-1 was determined by its ability to induce IL2 secretion by Jurkat human leukemic T cells. The expected ED50 is 0.3-0.9 μg/ml in the presence of PHA.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
vihvtk evkevatlsc ghnvsveela qtriywqkek kmvltmmsgd mniwpeyknr tifditnnls ivilalrpsd egtyecvvlk yekdafkreh laevtlsvka dfptpsisdf eiptsnirri icstsggfpe phlswlenge elnainttvs qdpetelyav sskldfnmtt nhsfmcliky ghlrvnqtfn wnttkqehfp dniegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgkh hhhhh
Country of Origin
USA
References
1. Abbas, A. K. et al. (1999) Nature Rev. Med. 5:1345
2. Sharpe, A. H. et al. (2002) Nature Rev. Immunol. 2:116
3. Chang, T. T. et al. (2002) Curr. Dir. Autoimmun. 5:113
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
