Recombinant Mouse B7-Homology 2 (B7-H1)
Purified No Carrier Protein
(B7RP-1, B7h, LICOS, GL50)
| Prod. No.: | B536 |
| Source | NSO Cells |
| Pkg. Size: | 1 mg, 100 µg |
| Storage: | -20°C to -70°C Detailed storage instructions below. |
Description
Background:
B7-H2, also known as ICOSL and B7RP-1, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains. B7-H2 is expressed on B cells and macrophages. It binds to ICOS on activated T cells and thus delivers a positive costimulatory signal for optimal T cell function (1). ICOS and B7RP-I do not interact with proteins in the CD28-B7 pathway (2). The structural features of B7-H2 are crucial for its costimulatory function. B7-H2 constructs lacking either the IgC or IgV domain demonstrates that receptor binding is mediated solely by the IgV domain but requires the IgC domain for maintaining the structural integrity of the protein (3).
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse B7-H2 is Mr 53 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 95 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Amino Acid Sequence
etev gamvgsnvvl scidphrrhf nlsglyvywq ienpevsvty ylpykspgin vdssyknrgh lsldsmkqgn fslylknvtp qdtqeftcrv fmntatelvk ileevvrlrv aanfstpvis tsdssnpgqe rtytcmskng ypepnlywin ttdnslidta lqnntvylnk lglydvistl rlpwtsrgdv lccvenvalh qnitsisqae sftgnntknp qethnnelkd iegrmdpksc dkthtcppcp apellggpsv flfppkpkdt lmisrtpevt cvvvdvshed pevkfnwyvd gvevhnaktk preeqynsty rvvsvltvlh qdwlngkeyk ckvsnkalpa piektiskak gqprepqvyt lppsrdeltk nqvsltclvk gfypsdiave wesngqpenn ykttppvlds dgsfflyskl tvdksrwqqg nvfscsvmhe alhnhytqks lslspgk
Country of Origin
USA
References
1. Wiendl, H. et al. (2003) Brain 126:1026
2. Yoshinaga, S. K. et al. (1999) Nature 402:827
3. Chattopadhyay, K. J. et al. (2006) Immunol. 177:3920
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
