Anti-Human B7-H1 (Antigen Aff. Pur.)

Product Datasheet

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Goat Anti-Human B7-Homolog 1 (B7-H1)

Antigen Affinity Purified

(B7-H, MGC142294, MGC142296, PD-L1, PDCD1L1, PDCD1LG1, PDL1)

Polyclonal Antibody

Prod. No.:	B560
Host:	Goat
Pkg. Size:	1 mg, 100 µg
Storage:	-20°C to -70°C Detailed storage instructions below.

Description

Specificity:

Goat Anti-Human B7-Homolog 1 (B7-H1) recognizes Human B7-H1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:

Human B7 homolog 1 (B7-H1), also called programmed cell death 1 ligand 1 (PDCD1L1) and programmed death ligand 1 (PDL1), is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PDL2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, their extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20 - 25% amino acid identity. Human and mouse B7-H1 share approximately 70% amino acid sequence identity. B7-H1 has been identified as one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors.

B7-H1 is found in a variety of non-lympoid parenchymal organs, including the heart, placenta, skeletal muscle and lung and has also been detected in most human cancers. On previously activated T cells, B7-H1 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. However, a costimulatory function for the PD-1 ligands on resting T cells has also been reported.

Known Species Cross Reactivity: Hu+

Immunogen

Purified Recombinant Human B7-H1 (>98%)

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Reconstitution

We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1.0 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.

Endotoxin

<0.1 EU/µg as determined by the LAL method

Storage and Stability

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Reported Applications

Indirect ELISA, WB

Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human B7-H1 is approximately 0.1 ng/well.

Western Blotting: To detect Human B7-H1 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human B7-H1 is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human B7-H1 (Leinco Prod. No.: B530).

Each investigator should determine their own optimal working dilution for specific applications.

Country of Origin

USA