Anti-Mouse cluster Of Differentiation 4 (CD4)
Purified Low Endotoxin Functional Formulation
(CD4mut, L3T4, Ly-4, Cd4, CD4 Antigen)
Monoclonal Antibody
| Prod. No.: | C1333 |
| Clone: | GK1.5 |
| Isotype: | Rat IgG2b κ |
| Conc.: | 1.0 - 5.0 mg/ml |
| Pkg. Size: | Custom, 50 µg, 0.5 mg |
| Storage: | Sterile 2-8°C Detailed storage instructions below. |
Description
Specificity:
Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Background:
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.1
Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purity
>98% by SDS Page and HPLC
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), FC, In vivo Depletion, B
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD4. A suggested method would be to stain cells expressing CD4 with 0.25 µg per 1 x 10
6 cells in a total staining volume of 100 µl followed by Goat F(ab')
2 Anti-Rat IgG, Fc (Gamma) Fragment Specific-R-phycoerythrin (
Leinco Prod. No.: R485).
Blocking: Anti-Mouse CD4 (clone GK1.5) is useful for In vitro and In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation.1,5
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Hendrickson WA et al. (1994) Structure 2: 59
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
