Anti-Mouse cluster Of Differentiation 4 (CD4)
Purified No Carrier Protein
(CD4mut, L3T4, Ly-4, Cd4, CD4 Antigen)
Monoclonal Antibody
| Prod. No.: | C1439 |
| Clone: | 131922 |
| Isotype: | Rat IgG2b |
| Pkg. Size: | 1 mg, 500 µg |
| Storage: | -20°C to -70°C Detailed storage instructions below. |
Description
Specificity:
Rat Anti-Mouse CD4 (Clone 131922) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.1
Host Species
Rat
Immunogen
NS0-Derived Recombinant Mouse CD4 Extracellular Domain
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Rat IgG (H+L)-Horseradish Peroxidase (
Leinco Prod. No.: R380). The detection sensitivity of this indirect ELISA for Mouse CD4 is approximately 25 ng/well.
Western Blotting: To detect Mouse CD4 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (
Leinco Prod. No.: R377) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (
Leinco Prod. No.: T343). The detection limit for Mouse CD4 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Hendrickson WA et al. (1994) Structure 2: 59
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
