Anti-Mouse CD5
Fluorescein (FITC)
Monoclonal Antibody
| Prod. No.: | C1529 |
| Clone: | 53-7.3 |
| Isotype: | Rat IgG2a |
| Pkg. Size: | 1 mg, 100 Tests |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Rat Anti-Mouse CD5 (Clone 53-7.3) recognizes an epitope on Mouse CD5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.
Reporter Molecule:
Fluorescein (FITC) is a small fluorescent organic molecule with a molecular weight of 473 daltons with an absorbance maximum of ~492 nm and an emission maximum of ~518 nm.
Host Species
Rat
Formulation
This monoclonal antibody is conjugated to fluorescein and formulated in modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 0.1% sodium azide as a preservative.
Storage and Stability
This Fluorescein (FITC) conjugate is stable for at least twelve months when stored at 2-8°C protected from light. Do not freeze.
Reported Applications
FC
Flow Cytometry: This Fluorescein (FITC) conjugate has been pre-diluted to use approximately 10 µl per test when staining cells for flow cytometry. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 105 continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes and resuspended in 200-400 µl of 1X PBS.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Leukocyte Typing VI (1995) Oxford University Press
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
