Anti-Human CD71
Biotin
(Transferrin Receptor)
Monoclonal Antibody
| Prod. No.: | C164 |
| Clone: | T56/14 |
| Isotype: | Mouse IgG1 |
| Conc.: | 0.1 mg/ml |
| Pkg. Size: | 50 µg, 100 µg, 200 µg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Mouse Anti-Human CD71 (Clone T56/14) recognizes Human CD71 transferrin receptor (Mr 190 kDa). This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
The transferrin receptor is a cell surface glycoprotein related to cell proliferation. Up-regulation of the CD71 antigen is associated with actively dividing cells.1 Transferrin receptors are also present on reticulocytes, but are lost during maturation into erythrocytes.2
Background:
Anti-transferrin receptor may be used to identify activated lymphocytes. The CD71 antibody may be used in studies of erythropoiesis.
Host Species
Mouse
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD71. A suggested method would be to stain cells expressing CD71 with Anti-Human CD71 at ≤1 µg per 1.0 X 10
6 cells in a 100 µl total staining volume, followed by UltraAvidin™- R-phycoerythrin (R-PE) (
Leinco Prod. No.: A104).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Trowbridge, S. I. et al. (1981) Proc. Nat'l. Acad. Sci. 78:3039
2. Iacopetta, B. J. et al. (1983) J. Histochem. Cytochem. 31:336
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
