Anti-Human CD71
R-phycoerythrin (R-PE)
(Transferrin Receptor)
Monoclonal Antibody
| Prod. No.: | C166 |
| Clone: | T56/14 |
| Isotype: | Mouse IgG1 |
| Conc.: | 0.1 mg/ml |
| Pkg. Size: | 50 µg, 100 µg, 200 µg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Mouse Anti-Human CD71 (Clone T56/14) recognizes Human CD71 transferrin receptor (Mr 190 kDa). This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
The transferrin receptor is a cell surface glycoprotein related to cell proliferation. Up-regulation of the CD71 antigen is associated with actively dividing cells.1 Transferrin receptors are also present on reticulocytes, but are lost during maturation into erythrocytes.2
Background:
Anti-transferrin receptor may be used to identify activated lymphocytes. The CD71 antibody may be used in studies of erythropoiesis.
Reporter Molecule:
R-phycoerythrin (R-PE) has a molecular weight of 240 kDa and is purified from red algae. R-PE is an intensely bright phycobiliprotein with an absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm.
Host Species
Mouse
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
FC: This antibody can be used at a concentration of ≤1 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Trowbridge, S. I. et al. (1981) Proc. Nat'l. Acad. Sci. 78:3039
2. Iacopetta, B. J. et al. (1983) J. Histochem. Cytochem. 31:336
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
