Anti-Mouse cluster Of Differentiation 4 (CD4)
Purified
(CD4mut, L3T4, Ly-4, Cd4, CD4 Antigen)
Monoclonal Antibody
| Prod. No.: | C211 |
| Clone: | GK1.5 |
| Isotype: | Rat IgG2b κ |
| Conc.: | 0.5 mg/ml |
| Pkg. Size: | 0.5 mg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Background:
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.1
Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), FC, IP, FA
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD4. A suggested method would be to stain cells expressing CD4 with ≤0.125 µg per 1 x 10
6 cells in a total staining volume of 100 µl followed by Goat F(ab')
2 Anti-Rat IgG, Fc (Gamma) Fragment Specific-R-phycoerythrin (
Leinco Prod. No.: R485).
Functional Assay: Anti-Mouse CD4 (clone GK1.5) is useful for
In vitro and
In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation.
1,5 It is recommend to use Anti-Mouse CD4 Purified Low Endotoxin Functional Formulation (
Leinco Prod. No.: C1333) for Functional Assays.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Hendrickson WA et al. (1994) Structure 2: 59
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
