Anti-Mouse CD90
Fluorescein (FITC)
(Thy-1)
Monoclonal Antibody
| Prod. No.: | C281 |
| Clone: | HK2.1 |
| Isotype: | Rat IgG2c |
| Conc.: | 0.5 mg/ml |
| Pkg. Size: | 0.5 mg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Rat Anti-Mouse CD90 (Clone HK2.1) recognizes the Thy-1.1 and Thy-1.2 alloantigens of all mouse strains tested. This antibody is reported to stimulate mouse T-cell proliferation.1
Antigen Distribution:
Anti-Mouse CD90 (Thy-1) is present on thymocytes, most peripheral T-lymphocytes, some intraepithelial T-lymphocytes, epithelial cells, fibroblasts, neurons and hematopoietic stem cells, but not B-cells of most mice tested.1,2
Reporter Molecule:
Fluorescein (FITC) is a small fluorescent organic molecule with a molecular weight of 473 daltons with an absorbance maximum of ~492 nm and an emission maximum of ~518 nm.
Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: This antibody can be used at a concentration of ≤ 1.0 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Ledbetter, J. A. et al. (1979) Immunol. Rev. 47:63
2. Ledbetter, J. A. et al. (1980) J. Exp. Med. 152:280
3. Lancki, D. W. et al. (1984) Immunol. Rev. 81:65
Related Products
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
