Anti-Mouse CD90
Biotin
(Thy-1.2)
Monoclonal Antibody
| Prod. No.: | C284 |
| Clone: | 30-H12 |
| Isotype: | Rat IgG2b |
| Conc.: | 0.5 mg/ml |
| Pkg. Size: | 0.5 mg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Rat Anti-Mouse CD90 (Clone 30-H12) recognizes the Thy-1.2 leukocyte marker.
Antigen Distribution:
Thy-1.2 is present on thymocytes, peripheral T-cells, cells of granulocytic lineage, early hematopoietic progenitors, epithelial cells, fibroblasts and neurons. It is found in most mouse strains except AKR/J, A, Thy-1.1 and B6.PL (74NS) expressing Thy-1.1.
Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC, WB
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD90. A suggested method would be to stain cells expressing CD90 with 1.0 µg per 1.0 x 10
6 cells in a total staining volume of 100 µl followed by UltraAvidin™- R-phycoerythrin (R-PE) (
Leinco Prod. No.: A104).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Ledbetter, J. and Herzenberg, L. (1979) Immunol. Rev. 47:63
2. Unkeless, J. C. (1979) J. Exp. Med. 150:580
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
