Anti-Mouse CD90
Purified Low Endotoxin Functional Formulation
(Thy)
Monoclonal Antibody
| Prod. No.: | C335 |
| Clone: | HK2.1 |
| Isotype: | Rat IgG2c |
| Conc.: | 0.5 mg/ml |
| Pkg. Size: | 0.5 mg |
| Storage: | Sterile 2-8°C Detailed storage instructions below. |
Description
Specificity:
Rat Anti-Mouse CD90 (Clone HK2.1) recognizes the Thy-1.1 and Thy-1.2 alloantigens of all mouse strains tested. This antibody is reported to stimulate mouse T-cell proliferation.1
Antigen Distribution:
Anti-Mouse CD90 (Thy-1) is present on thymocytes, most peripheral T-lymphocytes, some intraepithelial T-lymphocytes, epithelial cells, fibroblasts, neurons and hematopoietic stem cells, but not B-cells of most mice tested.1,2
Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purity
>98% by SDS Page and HPLC
Endotoxin
<1.0 EU/µg as determined by the LAL method
Storage and Stability
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), FC, FA
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD90. A suggested method would be to stain cells expressing CD90 with 1 µg per 1 x 10
6 cells in a total staining volume of 100 µl followed by Goat F(ab')
2 Anti-Rat IgG, Fc (Gamma) Fragment Specific-R-phycoerythrin (
Leinco Prod. No.: R485).
Functional Assay: Anti-Mouse CD90 (Thy-1) (clone HK2.1) has been implicated in activation of T-cells.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Ledbetter, J. A. et al. (1979) Immunol. Rev. 47:63
2. Ledbetter, J. A. et al. (1980) J. Exp. Med. 152:280
3. Lancki, D. W. et al. (1984) Immunol. Rev. 81:65
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
