Anti-Mouse CD4-Allophycocyanin

Product Datasheet

www.leinco.com

excellence in early discovery research

Anti-Mouse cluster Of Differentiation 4 (CD4)

Allophycocyanin (APC)

(CD4mut, L3T4, Ly-4, Cd4, CD4 Antigen)

Monoclonal Antibody

Prod. No.:	C338
Clone:	GK1.5
Isotype:	Rat IgG_2b
Conc.:	0.1 mg/ml
Pkg. Size:	0.1 mg
Storage:	2-8°C Detailed storage instructions below.

Description

Specificity:

Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.

Background:

CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.¹

Reporter Molecule:

Allophycocyanin (APC) has a molecular weight of 104 kDa and is purified from green algae. APC is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Known Species Cross Reactivity: Ms+

Host Species

Rat

Formulation

This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Stability

This APC conjugate is stable when stored at 2-8°C. Do not freeze.

Reported Applications

IP, FC

Flow Cytometry: This APC conjugate can be used at a concentration of ≤ 0.125 µg per 100 µl of whole blood (or per 1.0 x 10⁶ cells in a 100 µl total staining volume).

Each investigator should determine their own optimal working dilution for specific applications.

Country of Origin

USA

References

1. Hendrickson WA et al. (1994) Structure 2: 59

Related Products

Anti-Human CD4 Purified (Leinco Prod. No.: C121)
Anti-Human CD4 Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C367)
Anti-Human CD4-Biotin (Leinco Prod. No.: C122)
Anti-Human CD4-R-phycoerythrin (R-PE) (Leinco Prod. No.: C124)
Anti-Human CD4-Allophycocyanin (APC) (Leinco Prod. No.: C310)
Anti-Human CD4-Fluorescein (Leinco Prod. No.: C402)
Anti-Human CD4-R-phycoerythrin (Leinco Prod. No.: C403)
Anti-Mouse CD4-Biotin (Leinco Prod. No.: C212)
Anti-Mouse CD4-Fluorescein (Leinco Prod. No.: C213)
Anti-Mouse CD4-R-phycoerythrin (Leinco Prod. No.: C214)
Recombinant Human CD4 (Soluble) (Leinco Prod. No.: C1317)
Anti-Human CD4-Fluorescein (FITC) (Leinco Prod. No.: C123)
Anti-Mouse CD4 Purified (Leinco Prod. No.: C211)
Anti-Mouse CD4 Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C1333)
Anti-Mouse CD4 (Leinco Prod. No.: C1439)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

359 Consort Drive | St. Louis, MO 63011 | (800) 538-1145 | (636) 527-5545 fax