Anti-Human CD71
Purified Low Endotoxin Functional Formulation
(Transferrin Receptor)
Monoclonal Antibody
| Prod. No.: | C371 |
| Clone: | T56/14 |
| Isotype: | Mouse IgG1 |
| Conc.: | 1.0 - 5.0 mg/ml |
| Pkg. Size: | 100 µg, 500 µg, 1 mg |
| Storage: | Sterile 2-8°C Detailed storage instructions below. |
Description
Specificity:
Mouse Anti-Human CD71 (Clone T56/14) recognizes Human CD71 transferrin receptor (Mr 190 kDa). This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
The transferrin receptor is a cell surface glycoprotein related to cell proliferation. Up-regulation of the CD71 antigen is associated with actively dividing cells.1 Transferrin receptors are also present on reticulocytes, but are lost during maturation into erythrocytes.2
Background:
Anti-transferrin receptor may be used to identify activated lymphocytes. The CD71 antibody may be used in studies of erythropoiesis.
Host Species
Mouse
Formulation
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Storage and Stability
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
FC
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD71. A suggested method would be to stain cells expressing CD71 with Anti-Human CD71 at ≤1 µg per 1.0 X 10
6 cells in a 100 µl total staining volume, followed by Goat F(ab')
2 Anti-Mouse IgG (H&L)-R-phycoerythrin (
Leinco Prod. No.: M100).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Trowbridge, S. I. et al. (1981) Proc. Nat'l. Acad. Sci. 78:3039
2. Iacopetta, B. J. et al. (1983) J. Histochem. Cytochem. 31:336
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
