Recombinant Human Follistatin (FST)
Purified No Carrier Protein
(FS, Activins-Binding Protein, FSH-Suppressing Protein
)
| Prod. No.: | F168 |
| Source | Sf 21 Insect Cells |
| Pkg. Size: | 1 mg, 25 µg |
| Storage: | -20°C to -70°C Detailed storage instructions below. |
Description
Background:
Follistatin (FST) is a glycosylated single-chain protein. It specifically inhibits the secretion of FSH from the pituitary.1 Follistatin/activin-binding protein binds to heparan sulfate side chains of proteoglycans on the granulosa cell surface to regulate the various actions of activin.2 Follistatin regulates a variety of biological functions, including cell proliferation, differentiation, and apoptosis. Follistatin plays a critical role in the production of multiple-organ metastasis, predominantly by inhibiting the angiogenesis.3
Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Human FS is Mr 31 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 40 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human FS was determined by its ability to neutralize Activin-induced bioactivity on K562 cells (erythroid differentiation). The expected ED50 for this effect is typically 0.1 - 0.4 μg/ml in the presence of 7.5 ng/ml of rhActivin A.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
mg ncwlrqakng rcqvlyktel skeeccstgr lstswteedv ndntlfkwmi fnggapncip cketcenvdc gpgkkcrmnk knkprcvcap dcsnitwkgp vcgldgktyr necallkarc keqpelevqy qgrckktcrd vfcpgsstcv vdqtnnaycv tcnricpepa sseqylcgnd gvtyssachl rkatcllgrs iglayegkci kakscediqc tggkkclwdf kvgrgrcslc delcpdsksd epvcasdnat yasecamkea acssgvllev khsgscnsis edteeeeed
Country of Origin
USA
References
1. Ling N et al. (1989) Mol Endocrinol. 3: 651
2. Sugino H et al. (1991) J Biol Chem. 266: 19432
3. Sone S et al. (2008) Clin Cancer Res. 14: 660
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
