Donkey F(ab')2 Anti-Goat IgG (H&L)
Texas Red
Secondary Antibody
Adsorbed Against: Ms., Hu., Rt., Rb., Hs., GP., CK., Sy. Hms.
| Prod. No.: | G615 |
| Conc.: | 1.5 mg/ml |
| Pkg. Size: | 0.3 mg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Donkey F(ab')2 Anti-Goat IgG (H&L) recognizes Goat IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.
Reporter Molecule:
Texas Red is a small long wavelength fluorescent molecule with a molecular weight of 625 daltons with an absorbance maximum of ~589 nm and an emission maximum of ~615 nm.
Preparation:
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Host Species
Donkey
Immunogen
Purified Goat IgG (H&L) (>98%)
Formulation
This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
Reconstitution
The lyophilized secondary antibody Texas Red conjugate should be reconstituted in 0.25 ml of deionized water. Gently vortex until the solution is clear. Centrifuge the conjugate if not completely clear and free of particulates. After reconstitution, add an equal volume of ACS grade glycerol bringing the final concentration to 50% v/v.
Expiration Date
One year from date of reconstitution
Storage and Stability
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Texas Red conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.
Applications and Suggested Working Dilutions
It is recommended that working dilutions be prepared fresh daily. IF: 1:50 - 1:200Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Country of Origin
USA
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