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Donkey Anti-Goat IgG (H&L)
DyLight® 488
Secondary Antibody
Adsorbed Against: Ms., Hu., Rt., Rb., Hs., GP., CK., Sy. Hms.
SizeQuantityTotal
0.5 mg
Prod. No.:G711
Conc.:1.5 mg/ml
Pkg. Size:0.5 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Donkey Whole Molecule Anti-Goat IgG (H&L) recognizes Goat IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:
DyLightŪ 488 (Green) is a member of the new DyLightŪ family of fluorescent dyes displaying superior brightness and photostability. DyLightŪ 488 has an absorbance maximum of ~493 nm and an emission maximum of ~518 nm.

DyLightŪ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Host Species
Donkey
Immunogen
Purified Goat IgG (H&L) (>98%)
Formulation
This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
Reconstitution
The lyophilized secondary antibody DyLight® 488 conjugate should be reconstituted in 0.4 ml of deionized water. Gently vortex until the solution is clear. Centrifuge the conjugate if not completely clear and free of particulates. After reconstitution, add an equal volume of ACS grade glycerol bringing the final concentration to 50% v/v.
Expiration Date
One year from date of reconstitution
Storage and Stability
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted DyLight® 488 conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.
Applications and Suggested Working Dilutions
It is recommended that working dilutions be prepared fresh daily.

FC: 1:100 - 1:800
IF: 1:100 - 1:800

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Country of Origin
USA
Related Products
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Donkey Anti-Goat IgG (H&L)-Biotin (Leinco Prod. No.: G598)
Donkey Anti-Goat IgG (H&L)-Cy5 (Leinco Prod. No.: G599)
Donkey Anti-Goat IgG (H&L)-Texas Red (Leinco Prod. No.: G600)
Donkey Anti-Goat IgG (H&L)-Rhodamine Red (Leinco Prod. No.: G601)
Donkey Anti-Goat IgG (H&L)-Rhodamine TRITC (Leinco Prod. No.: G602)
Donkey Anti-Goat IgG (H&L)-Cy3 (Leinco Prod. No.: G603)
Donkey Anti-Goat IgG (H&L)-FITC (Leinco Prod. No.: G604)
Donkey Anti-Goat IgG (H&L)-Cy2 (Leinco Prod. No.: G605)
Donkey Anti-Goat IgG (H&L)-AMCA (Leinco Prod. No.: G606)
Donkey Anti-Goat IgG (H&L)-Purified No Carrier Protein (Leinco Prod. No.: G607)
Donkey F(ab')2 Anti-Goat IgG (H&L)-Purified No Carrier Protein (Leinco Prod. No.: G608)
Donkey F(ab')2 Anti-Goat IgG (H&L)-AMCA (Leinco Prod. No.: G609)
Donkey F(ab')2 Anti-Goat IgG (H&L)-Cy2 (Leinco Prod. No.: G610)
Donkey F(ab')2 Anti-Goat IgG (H&L)-FITC (Leinco Prod. No.: G611)
Donkey F(ab')2 Anti-Goat IgG (H&L)-Cy3 (Leinco Prod. No.: G612)
Donkey F(ab')2 Anti-Goat IgG (H&L)-Rhodamine TRITC (Leinco Prod. No.: G613)
Donkey F(ab')2 Anti-Goat IgG (H&L)-Rhodamine Red-X (Leinco Prod. No.: G614)
Donkey F(ab')2 Anti-Goat IgG (H&L)-Texas Red (Leinco Prod. No.: G615)
Donkey F(ab')2 Anti-Goat IgG (H&L)-Cy5 (Leinco Prod. No.: G616)
Donkey F(ab')2 Anti-Goat IgG (H&L)-Biotin (Leinco Prod. No.: G617)
Donkey F(ab')2 Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G618)
Donkey F(ab')2 Anti-Goat IgG (H&L)-Alk Phos (Leinco Prod. No.: G619)
Donkey F(ab')2 Anti-Goat IgG (H&L)-Allophycocyanin (APC) (Leinco Prod. No.: G642)
Donkey F(ab')2 Anti-Goat IgG (H&L)-R-phycoerythrin (R-PE) (Leinco Prod. No.: G641)
Donkey Anti-Goat IgG (H&L) - DyLight® 549 (Leinco Prod. No.: G712)
Donkey Anti-Goat IgG (H&L) - DyLight® 594 (Leinco Prod. No.: G713)
Donkey Anti-Goat IgG (H&L) - DyLight® 649 (Leinco Prod. No.: G714)
Donkey F(ab')2 Anti-Goat IgG (H&L) - DyLight® 488 (Leinco Prod. No.: G720)
Donkey F(ab')2 Anti-Goat IgG (H&L) - DyLight® 549 (Leinco Prod. No.: G721)
Donkey F(ab')2 Anti-Goat IgG (H&L) - DyLight® 594 (Leinco Prod. No.: G722)
Donkey F(ab')2 Anti-Goat IgG (H&L) - DyLight® 649 (Leinco Prod. No.: G723)
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