Adsorbed Against: Hu., Bv., Hs.
Fluorescein (FITC) is a small fluorescent organic molecule with a molecular weight of 473 daltons with an absorbance maximum of ~492 nm and an emission maximum of ~518 nm.
This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
The lyophilized secondary antibody Fluorescein (FITC) conjugate should be reconstituted in 0.75 ml of deionized water. Gently vortex until the solution is clear. Centrifuge the conjugate if not completely clear and free of particulates. After reconstitution, add an equal volume of ACS grade glycerol bringing the final concentration to 50% v/v.
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Fluorescein (FITC) conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.
A working dilution of 1:50 - 1:200 is suitable for most applications.