Adsorbed Against: Hu., Bv., Hs.
Rhodamine RED X is a small fluorescent organic molecule with an absorbance maximum of 570 nm and an emission maximum of 590 nm.
This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
The lyophilized secondary antibody Rhodamine RED X conjugate should be reconstituted in 0.75 ml of deionized water. Gently vortex until the solution is clear. Centrifuge the conjugate if not completely clear and free of particulates. After reconstitution, add an equal volume of ACS grade glycerol bringing the final concentration to 50% v/v.
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Rhodamine RED X conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.
A working dilution of 1:50 - 1:200 is suitable for most applications.