This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Goat

Purified Mouse IgG (H&L) (>98%)

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

The lyophilized secondary antibody Horseradish Peroxidase (HRPO) conjugate should be reconstituted in 0.5 ml of deionized water. Gently vortex until the solution is clear. Centrifuge the conjugate if not completely clear and free of particulates. After reconstitution, add an equal volume of ACS grade glycerol bringing the final concentration to 50% v/v.

One year from date of reconstitution

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. Caution: This horseradish peroxidase conjugate does not contain preservatives. To avoid microbial contamination, store the reconstituted conjugate containing glycerol at -20°C in a manual defrost freezer.

WB: 1:5,000 - 1:100,000
WB Chemiluminescent: :10,000 - 1:200,000
ELISA: 1:5,000 - 1:100,000
IHC: 1:500 - 1:5,000

USA

Goat F(ab')₂ Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M100)
Goat F(ab')₂ Anti-Mouse IgG (H&L) Allophycocyanin (Leinco Prod. No.: M101)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Fluorescein FITC (Leinco Prod. No.: M102)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M103)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Alkaline Phosphatase (Leinco Prod. No.: M104)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Biotin (Leinco Prod. No.: M105)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Texas Red (Leinco Prod. No.: M107)
Goat Anti-Mouse IgG (H&L)-Purified No Carrier Protein (Leinco Prod. No.: M111)
Goat F(ab')₂ Anti-Mouse IgG (H&L) Purified (Leinco Prod. No.: M112)
Goat Anti-Mouse IgG (H&L)-Fluorescein (Leinco Prod. No.: M113)
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
Goat Anti-Mouse IgG (H&L) Alkaline Phosphatase (Leinco Prod. No.: M115)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Rhodamine TRITC (Leinco Prod. No.: M116)
Goat Anti-Mouse IgG (H&L) Purified No Carrier Protein (Leinco Prod. No.: M1039)
Goat Anti-Mouse IgG (H&L)-Rhodamine TRITC (Leinco Prod. No.: M1064)
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M1087)
Goat Anti-Mouse IgG (H&L)-Alkaline Phosphatase (Leinco Prod. No.: M290)
Goat Anti-Mouse IgG (H&L)-Biotin (Long Chain Spacer) (Leinco Prod. No.: M313)
Goat Anti-Mouse IgG (H&L)-Texas Red (Leinco Prod. No.: M336)
Goat Anti-Mouse IgG (H&L)-FITC (Leinco Prod. No.: M359)
Goat Anti-Mouse IgG (H&L)-AMCA (Leinco Prod. No.: M392)
Goat Anti-Mouse IgG (H&L)-Cy3 (Leinco Prod. No.: M415)
Goat Anti-Mouse IgG (H&L)-Cy5 (Leinco Prod. No.: M440)
Goat Anti-Mouse IgG (H&L)-Cy2 (Leinco Prod. No.: M485)
Goat Anti-Mouse IgG (H&L)-Rhodamine Red-X (Leinco Prod. No.: M510)
Goat F(ab')₂ Anti-Mouse IgG (H+L)-Cy3 (Leinco Prod. No.: M423)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Cy5 (Leinco Prod. No.: M448)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Cy2 (Leinco Prod. No.: M493)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Rhodamine Red-X (Leinco Prod. No.: M518)
Goat Anti-Mouse IgG (H&L)-Purified No Carrier Protein (Leinco Prod. No.: M1194)
Goat Anti-Mouse IgG (H&L)-AMCA (Leinco Prod. No.: M1195)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Purified No Carrier Protein (Leinco Prod. No.: M1173)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-AMCA (Leinco Prod. No.: M1174)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Cy2 (Leinco Prod. No.: M1175)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-FITC (Leinco Prod. No.: M1176)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Rhodamine TRITC (Leinco Prod. No.: M1178)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Rhodamine Red-X (Leinco Prod. No.: M1179)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Cy5 (Leinco Prod. No.: M1180)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Texas Red (Leinco Prod. No.: M1181)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Biotin (Leinco Prod. No.: M1182)
Goat F(ab')₂ Anti-Mouse IgG (H&L)-Alk Phos (Leinco Prod. No.: M1184)
Goat Anti-Mouse IgG (H&L)-AMCA (Leinco Prod. No.: M1185)
Goat Anti-Mouse IgG (H&L)-Rhodamine TRITC (Leinco Prod. No.: M1186)
Goat Anti-Mouse IgG (H&L)-Rhodamine Red-X (Leinco Prod. No.: M1187)
Goat Anti-Mouse IgG (H&L)-Biotin (Leinco Prod. No.: M1188)
Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189)
Goat Anti-Mouse IgG (H&L)-Allophycocyanin (Leinco Prod. No.: M1191)
Goat Anti-Mouse IgG (H&L)-Alkaline Phosphatase (Premiere Grade) (Leinco Prod. No.: M1192)
Goat Anti-Mouse IgG (H&L)-Cy2 (Leinco Prod. No.: M1196)
Goat Anti-Mouse IgG (H&L)-FITC (Leinco Prod. No.: M1197)
Goat Anti-Mouse IgG (H&L)-Cy3 (Leinco Prod. No.: M1198)
Goat Anti-Mouse IgG (H&L)-Texas Red (Leinco Prod. No.: M1199)
Goat Anti-Mouse IgG (H&L)-Rhodamine TRITC (Leinco Prod. No.: M1200)
Goat Anti-Mouse IgG (H&L)-Rhodamine Red-X (Leinco Prod. No.: M1201)
Goat Anti-Mouse IgG (H&L)-Texas Red (Leinco Prod. No.: M1202)
Goat Anti-Mouse IgG (H&L)-Cy5 (Leinco Prod. No.: M1203)
Goat Anti-Mouse IgG (H&L)-Biotin (Leinco Prod. No.: M1204)
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M1205)
Goat Anti-Mouse IgG (H&L)-Alkaline Phosphatase (Leinco Prod. No.: M1206)
Goat Anti-Mouse IgG (H&L)-Allophycocyanin (Leinco Prod. No.: M1258)
Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1259)
Goat Anti-Mouse IgG (H&L) - DyLight® 488 (Leinco Prod. No.: M1327)
Goat Anti-Mouse IgG (H&L) - DyLight® 549 (Leinco Prod. No.: M1328)
Goat Anti-Mouse IgG (H&L) - DyLight® 594 (Leinco Prod. No.: M1329)
Goat Anti-Mouse IgG (H&L) - DyLight® 649 (Leinco Prod. No.: M1330)
Goat Anti-Mouse IgG (H&L) - DyLight® 488 (Leinco Prod. No.: M1331)
Goat Anti-Mouse IgG (H&L) - DyLight® 549 (Leinco Prod. No.: M1332)
Goat Anti-Mouse IgG (H&L) - DyLight® 594 (Leinco Prod. No.: M1333)
Goat Anti-Mouse IgG (H&L) - DyLight® 649 (Leinco Prod. No.: M1334)
Goat F(ab')₂ Anti-Mouse IgG (H&L) - DyLight® 488 (Leinco Prod. No.: M1352)
Goat F(ab')₂ Anti-Mouse IgG (H&L) - DyLight® 549 (Leinco Prod. No.: M1353)
Goat F(ab')₂ Anti-Mouse IgG (H&L) - DyLight® 594 (Leinco Prod. No.: M1354)
Goat F(ab')₂ Anti-Mouse IgG (H&L) - DyLight® 649 (Leinco Prod. No.: M1355)