Recombinant Rat Platelet-Derived Growth Factor-BB (PDGF-BB)
Purified No Carrier Protein
(Glioma-Derived Growth Factor (GDGF), Osteosarcoma-Derived Growth Factor (ODGF), SIS, C-Sis, Pdgfb)
| Prod. No.: | P256 |
| Source | E. coli |
| Pkg. Size: | 1 mg, 50 µg |
| Storage: | -20°C to -70°C Detailed storage instructions below. |
Description
Background:
Platelet-derived growth factor-BB homodimers (PDGF-BB) activates all combinations of PDGF receptor subunits, whereas PDGF-AA acts specifically on PDGFR alpha.1 This constitutes a positive feed-back mechanism, which, for example, could serve to potentiate autocrine stimulation of growth.2 PDGF-BB plays an important role in the cellular metabolism of vascular wall by regulating the rate of macrophage-colony stimulating factor (M-CSF) production in vascular smooth muscle cells.3 PDGF-BB is also a potent wound-healing hormones that accelerate incisional repair.4 PDGF-BB is associated with excessive cell migration, proliferation and many growth-related diseases.5 It may be a new prognostic indicator for patients with colorectal cancer.6 PDGF-BB may also induce coronary atherosclerotic disease formation by stimulating coronary artery smooth muscle cell (CASMC) migration and proliferation through agent-specific regulation of oxidative status and utilization of different signal transduction pathways.5
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Rat PDGF-BB is Mr 25 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Rat PDGF-BB was determined by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts (Raines, E. W. et al., 1985, Methods in Enzymology 109:749-773). The expected ED50 for this effect is typically 1 - 3 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
slgslaaaep aviaecktrt evfqisrnli drtnanflvw ppcvevqrcs gccnnrnvqc rasqvqmrpv qvrkieivrk kpvfkkatvt ledhlackce tvvtprpvt
Country of Origin
USA
References
1. Josef Pfeilschifter et al. (2005) Am Society of Nephrol. 10: 1681
2. Claesson-Welsh L. et al. (1991) J Biol Chem. 266: 21138
3. Yamada N et al. (1992) J Biol Chem. 267: 15455
4. Mustoe TA et al. (1991) J Lab Clin Med. 117: 373
5. Bayraktutan U. et al. (2008) J Mol Cell Cardiol. 45: 198
6. Mori M et al. (2008) Ann Surg Oncol. 15: 2129
Related Products
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
