Goat F(ab')2 Anti-Rat IgG, Fc (Gamma) Fragment Specific
R-phycoerythrin (R-PE)
Secondary Antibody
Adsorbed Against: Hu., Bv., Hs.
| Prod. No.: | R485 |
| Conc.: | 0.5 mg/ml |
| Pkg. Size: | 0.5 mg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Goat F(ab')2 Anti-Rat IgG, Fc (Gamma) Fragment Specific recognizes Rat IgG, Fc (Gamma) Fragment Specific. This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.
Reporter Molecule:
R-phycoerythrin (R-PE) has a molecular weight of 240 kDa and is purified from red algae. R-PE is an intensely bright phycobiliprotein with an absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm.
Preparation:
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Host Species
Goat
Immunogen
Purified Rat IgG, Fc (Gamma) Fragment Specific (>98%)
Formulation
This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
Expiration Date
One year from date of reconstitution
Storage and Stability
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted R-phycoerythrin (R-PE) conjugate should be stored at 2-8°C. Do Not Freeze.
Applications and Suggested Working Dilutions
1:50 - 1:200 for most applications.
Country of Origin
USA
Related Products
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
