Recombinant Mouse Stromal Cell-Derived Factor-1 Alpha (SDF-1α)
Purified No Carrier Protein
(CXCL12, TLSF-a, Pre-B Cell Growth Stimulating Factor (PBSF))
| Prod. No.: | S501 |
| Source | E. coli |
| Pkg. Size: | 1 mg, 10 µg, 50 µg |
| Storage: | -20°C to -70°C Detailed storage instructions below. |
Description
Background:
SDF-1alpha (stromal cell-derived factor-1 alpha) is an alpha-chemokine for haematopoietic stem cells (HSC).1 SDF-1 alpha plays a role in two important processes of granule cell maturation - proliferation and migration - assisting in the achievement of appropriate cell number and position in the cerebellar cortex.2 SDF-1 alpha is also involved in the trafficking of hematopoietic progenitors and stem cells.3 It is expressed by liver cells and in liver diseases.4
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Mouse SDF-1α is Mr 8 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse SDF-1α was determined by its ability to chemoattract cultured human lymphocytes, and mouse BaF/3 cells transfected with human CXCR4. The expected ED50 for this effect is typically 3-9 ng/ml, and 0.15-0.6 ng/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
kpvslsyrc pcrffeshia ranvkhlkil ntpncalqiv arlknnnrqv cidpklkwiq eylekalnk
Country of Origin
USA
References
1. Ratajczak MZ. et al. (2003) Folia Histochem Cytobiol. 41: 13
2. Luster AD et al. (2001) Development 128: 1971
3. Vannier JP et al. (2002) Eur Cytokine Netw 12: 223
4. James Dickenson et al. (2008) Iran J Allergy Asthma Immunol. 7: 61
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
