Goat Anti-Mouse TROP-2
Antigen Affinity Purified
(Tumor-Associated Calcium Signal Transducer 2 (TACSTD2), EGP-1, GA733, GA733-1, M1S1, T16, GP50)
Polyclonal Antibody
| Prod. No.: | T412 |
| Host: | Goat |
| Pkg. Size: | 1 mg, 100 µg |
| Storage: | -20°C to -70°C Detailed storage instructions below. |
Description
Specificity:
Goat Anti-Mouse TROP-2 recognizes Mouse TROP-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Trop-2 is a cell surface glycoprotein1 that is highly expressed in a wide variety of epithelial cancers. There is little or no expression of Trop-2 in adult somatic tissue.2 Trop-2 is a novel calcium signal transducer3 involved in the regulation of cell- cell adhesion.4 It is an oncogene that has potential as a therapeutic target.2 It is a possible candidate gene for diagnosis and molecular target therapy of colorectal cancer.5
Known Species Cross Reactivity: Ms+
Immunogen
NSO-derived mouse TROP-2 extracellular domain
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1.0 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), FC, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (
Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Mouse TROP-2 is approximately 0.15 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (
Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (
Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (
Leinco Prod. No.: K107).
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TROP-2. A suggested method would be to stain cells expressing TROP-2 with 25 µg per 1 - 2.5 x 10
5 cells in a total staining volume of 200 µl followed by Donkey F(ab')
2 Anti-Goat IgG (H&L)-R-phycoerythrin (R-PE) (
Leinco Prod. No.: G641).
Western Blotting: To detect Mouse TROP-2 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (
Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (
Leinco Prod. No.: T343). The detection limit for Mouse TROP-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Colnaghi MI. i>et al. (1992) Hybridoma 11: 539
2. Michel L et al. (2008) Mol Cancer Ther. 7: 280
3. Alberti S et al. (1998) Int J Cancer 75: 324
4. M. Piantelli et al. (2007) J Clin Oncol. 25: 10510
5. Masaki Mori et al. (2006) clin Cancer Res. 12: 3057
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
