Anti-Mouse TROP-2
Purified No Carrier Protein
(Tumor-Associated Calcium Signal Transducer 2 (TACSTD2), EGP-1, GA733, GA733-1, M1S1, T16, GP50)
Monoclonal Antibody
| Prod. No.: | T424 |
| Clone: | 168215 |
| Isotype: | Rat IgG2b |
| Pkg. Size: | 1 mg, 500 µg |
| Storage: | -20°C to -70°C Detailed storage instructions below. |
Description
Specificity:
Rat Anti-Mouse TROP-2 (Clone 168215) recognizes an epitope on Mouse TROP-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Trop-2 is a cell surface glycoprotein1 that is highly expressed in a wide variety of epithelial cancers. There is little or no expression of Trop-2 in adult somatic tissue.2 Trop-2 is a novel calcium signal transducer3 involved in the regulation of cell- cell adhesion.4 It is an oncogene that has potential as a therapeutic target.2 It is a possible candidate gene for diagnosis and molecular target therapy of colorectal cancer.5
Host Species
Rat
Immunogen
Purified Recombinant Mouse TROP-2 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Goat Anti-Rat IgG (H+L)-Horseradish Peroxidase (
Leinco Prod. No.: R380). The detection sensitivity of this indirect ELISA for Mouse TROP-2 is approximately 2.0 ng/well.
Western Blotting: To detect Mouse TROP-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (
Leinco Prod. No.: R377) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (
Leinco Prod. No.: T343). The detection limit for Mouse TROP-2 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Colnaghi MI. i>et al. (1992) Hybridoma 11: 539
2. Michel L et al. (2008) Mol Cancer Ther. 7: 280
3. Alberti S et al. (1998) Int J Cancer 75: 324
4. M. Piantelli et al. (2007) J Clin Oncol. 25: 10510
5. Masaki Mori et al. (2006) clin Cancer Res. 12: 3057
Related Products
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
