Sandwich ELISA Protocol
An Enzyme-linked immunosorbent assay or Sandwich ELISA is a scientific technique used by researchers to detect the presence of an antibody or an antigen in a sample. An ELISA can also be used as a diagnostic tool in medicine. Leinco Technologies uses the ELISA as a quality control test to validate our antibodies and proteins.
This protocol provides an initial set of conditions; however, further optimization may be required on an individual basis.
Coating the Plate with Capture Antibody
Blocking the Plate
Add Antigen Coating Sample
Add the Detection Antibody
Add UltraAvidin-Horseradish Peroxidase Conjugate