Anti-Human FGF R2 (Clone 98725)

Anti-Human FGF R2 (Clone 98725)

Product No.: F1079

[product_table name="All Top" skus="F1079"]

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Clone
98725
Target
FGF R2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Fibroblast Growth Factor Receptor 2
Isotype
IgG1
Applications
ELISA Indirect
,
FC
,
N
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Sf 21- and NS0-Derived Recombinant Human FGF R2 Isoforms
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing FGF R2. A suggested method would be to stain cells expressing FGF R2 with 0.25 µg per 1 - 2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:M1189.
Western Blotting: To detect Human FGF R2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human FGF R2 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human FGF R2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human FGF R2 (at 4 ng/ml) is 3-8 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human FGF R2 (Clone 98725) recognizes an epitope on Human FGF R2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Details

PubMed
NCBI Gene Bank ID
Indirect Elisa Protocol
Flow Cytometry
N
General Western Blot Protocol
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Formats Available

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Prod No.
Description
F1079
Products are for research use only. Not for use in diagnostic or therapeutic procedures.