Anti-Human Lymphotoxin βR – Biotin

Anti-Human Lymphotoxin βR – Biotin

Product No.: L232

[product_table name="All Top" skus="L232"]

- -
- -
Target
Lymphotoxin βR
Product Type
Polyclonal Antibody
Alternate Names
Lymphotoxin Beta Receptor, CD18, D12S370, LT-BETA-R, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFRSF3
Applications
FC
,
WB

- -
- -
Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human Lymphotoxin βR (Accession # P36941)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Lymphotoxin βR. A suggested method would be to stain cells expressing Lymphotoxin βR with 2.5 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:A104.
Western Blotting: To detect Human Lymphotoxin βR this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:L201.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Lymphotoxin Beta Receptor (Lymphotoxin βR) recognizes Human Lymphotoxin βR. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Lymphotoxin beta receptor (LTBR), a receptor for lymphotoxin, is a member of the tumor necrosis factor (TNF) family of receptors. It is expressed on the surface of most cell types, including cells of epithelial and myeloid lineages. LTBR specifically binds the lymphotoxin membrane form (a complex of lymphotoxin-alpha and lymphtoxin-beta). The encoded protein and its ligand play a role in the development and organization of lymphoid tissue and tranformed cells. Activation of the encoded protein can trigger apoptosis. LTBR pathway is important in the development and maintenance of lymphoid structures. Blocking this pathway has proved beneficial in autoimmune diseases such as diabetes and rheumatoid arthritis.1

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Isine Bolstad, A. et al. (2009) Arthritis Research & Therapy 11: R24
Flow Cytometry
General Western Blot Protocol
- -
- -
Prod No.
Description
L232
Products are for research use only. Not for use in diagnostic or therapeutic procedures.