Anti-Human PDGF Rα – Biotin

Anti-Human PDGF Rα – Biotin

Product No.: P290

[product_table name="All Top" skus="P290"]

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Target
PDGF Rα
Product Type
Polyclonal Antibody
Alternate Names
PDGF Receptor Alpha, PDGFRA, CD140A, MGC74795, PDGFR2, Rhe-PDGFRA
Applications
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human PDGF Rα (Accession # P16234)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human PDGF Rα this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human PDGF Rα is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P253.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Platelet Derived Growth Factor α (PDGF Rα) recognizes Human PDGF Rα. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Platelet-derived growth factor receptor, alpha polypeptide, also known as PDGFRA, encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. A soluble form of the platelet-derived growth factor (PDGF) alpha receptor, designated sPDGF-R alpha is capable of binding PDGF ligand and can compete with cell-associated PDGF receptors for ligand binding.1

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Hart, CE. et al. (1993) J Biol Chem. 268: 9621
General Western Blot Protocol
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Prod No.
Description
P290
Products are for research use only. Not for use in diagnostic or therapeutic procedures.