Anti-Human CD120b (TNFR2)
Anti-Human CD120b (TNFR2)
Product No.: T457
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Clone 22210 Target TNFR2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Tumor Necrosis Factor Receptor II, TNFRSF1B, p75, CD120b, TBPII, TNF-R75, TNFBR, TNFR2, TNFR80, p75TNFR Isotype IgG1 Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human TNF RII (Accession # P20333) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831957 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T240 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T408. Western Blotting: To detect Human TNF RII this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TNF RII is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T408. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human TNF RII bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human TNF RII (at 0.3 ng/ml) is 0.5 - 1.5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Tumor Necrosis Factor Receptor Type II (TNF RII) (Clone 22210) recognizes an epitope on Human TNF RII. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Tumor necrosis factor receptor II (TNF-RII) is present on most cell types1 and is considered to play a prominent role in stimulation by TNF-alpha. TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. It is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells.2 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Zuckerman, KS. et al. (1998) Cancer Res. 58:2217. 2. Sheehan, KC. et al. (1995) J. Exp. Med. 181:607. Technical Protocols N  |
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T457 | |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
