Anti-Mouse PDGF Rα

Anti-Mouse PDGF Rα

Product No.: P263

[product_table name="All Top" skus="P263"]

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Target
PDGF R alpha
Product Type
Polyclonal Antibody
Alternate Names
Platelet-Derived Growth Factor Receptor Alpha, PDGFRA, CD140A, MGC74795, PDGFR2, Rhe-PDGFRA
Applications
IHC FFPE
,
N
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
NS0-derived mouse PDGF Rα extracellular domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse PDGF Rα this polyclonal antibody can be used at a concentration of 1 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse PDGF Rα is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P249.
Additional Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Mouse PDGF Rα bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse PDGF Rα (at 10 ng/ml) is 1-5 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse Platelet-Derived Growth Factor Receptor Alpha (PDGF Rα) recognizes Mouse PDGF Rα. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Platelet-derived growth factor receptor, alpha polypeptide, also known as PDGFRA, encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. A soluble form of the platelet-derived growth factor (PDGF) alpha receptor, designated sPDGF-R alpha is capable of binding PDGF ligand and can compete with cell-associated PDGF receptors for ligand binding.1

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Hart, CE. et al. (1993) J Biol Chem. 268: 9621
IHC FFPE
N
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.