Anti-Mouse TRAIL R2

Anti-Mouse TRAIL R2

Product No.: T474

[product_table name="All Top" skus="T474"]

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Target
TRAIL R2
Product Type
Polyclonal Antibody
Alternate Names
TNF-Related Apoptosis-Inducing Ligand Receptor 2, TNFRSF10B, CD262, DR5, KILLER, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9
Applications
ELISA Indirect
,
FC
,
N
,
WB

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Product Size

Data

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
NS0-Derived Recombinant Mouse TRAIL Receptor 2 Extracellular Domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TRAIL R2. A suggested method would be to stain cells expressing TRAIL R2 with 0.25 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:G641.
Western Blotting: To detect Mouse TRAIL R2 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T263. The detection limit for Mouse TRAIL R2 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse TRAIL R2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse TRAIL R2 (at 12 ng/ml) is 0.4 - 1.2 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse TNF-Related Apoptosis-Inducing Ligand Receptor 2 (TRAIL R2) recognizes Mouse TRAIL R2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Tumor necrosis factor-related apoptosis-inducing ligand Receptor 2 (TRAIL-R2) is a cell-surface receptor involved in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced cell-death signaling.1 The death ligand TRAIL bears high potential as a new anticancer agent, as binding to the death receptors TRAIL-R1 or TRAIL-R2 triggers apoptosis in most cancer cells.2 TRAIL-R2 has been shown to be associated with a decrease in the survival rates of breast cancer patients.3

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Yoo, NJ. et al. (2001) Oncogene 20: 399
2. Eberle, J. et al. (2005) J Investigative Dermatol. 125: 1010
3. Rimm, D. et al. (2005) Clin Cancer Research 11
Indirect Elisa Protocol
Flow Cytometry
N
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.