Anti-Mouse VEGF R2

Product No.: V141

[product_table name="All Top" skus="V141"]

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Clone
91201
Target
VEGF R2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
KDR, Flk-1, CD309, VEGFR, Vascular Endothelial Growth Factor Receptor 2
Isotype
IgG1
Applications
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse VEGF R2 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse VEGF R2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:V120.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Vascular Endothelial Growth Factor Receptor 2 (VEGF R2) (Clone 91201) recognizes an epitope on Mouse VEGF R2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Vascular endothelial growth factor receptor 2 (VEGF R2) is a transmembrane glycoprotein that is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration, and differentiation.1,2,3

Antigen Details

PubMed

References & Citations

1. Homqvist, K. et al. (2004) J Biol Chem. 279: 22267
2. Meyer, RD. et al. (2002) J Biol Chem. 277: 27081
3. Rahimi, N. et al. (2000) J Biol Chem. 275: 16986
General Western Blot Protocol

Formats Available

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Prod No.
Description
V141
Products are for research use only. Not for use in diagnostic or therapeutic procedures.