UltraAvidin™- APC Premium Grade

UltraAvidin™- APC Premium Grade

Product No.: A107

[product_table name="All Top" skus="A107"]

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Product Type
Avidin - Biotin

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Product Size
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Antibody Details

Product Details

Host Species
Chicken Egg White
Product Concentration
0.2 mg/ml
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Working Concentration
UltraAvidin-APC has been tested by flow cytometric analysis to detect biotinylated primary antibodies. A working dilution ≤ 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume) is suitable for most applications. However, each investigator should determine their own optimal working dilution for each specific research application.
Isoelectric Point
pI= 6.3 +/- 0.3
Specific Activity
11-17 µg biotin bound/ng UltraAvidin™
Excitation Laser
Red Laser (650 nm)
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Background
UltraAvidin™ is Leinco Technologies' trademark for a uniquely modified form of avidin which is isolated from chicken egg whites. With a molecular weight of 60,000, there are four identical subunits each capable of binding one molecule of biotin. UltraAvidin from Leinco Technologies has been de-glycosylated to prevent carbohydrate moieties from adhering to lectin-like receptors on the surface of cells, thus eliminating the possibility of false positives. Unlike native avidin, UltraAvidin has a near neutral pI which prevents electrostatic interactions with negatively charged serum or membrane proteins.

Recent studies have identified a universal recognition sequence in streptavidin which is similar to that found in several adhesion receptors.(1) It is a tripeptide sequence Arg-Tyr-Asp (RYD) which mimics the Arg-Gly-Asp (RGD) binding sequence of fibronectin. Strong interactions have been shown with streptavidin and cell surface molecules. This universal recognition sequence is not present in UltraAvidin. UltraAvidin may not significantly increase sensitivity in all research systems but will help assure specificity.

Antigen Details

References & Citations

1. Wilchek, M. et al. (1990) Biochem Biophys Res Commun. 170(3):1236-41.
2. Sullivan, W. et al. (1996) Genetics. 143(4):1629-42.
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Prod No.
Description
M1036
M1049
R344
H568
M356
H665
H617
M389
R433
R389
M1356
M1358
M1359
M310
M1084
M287
R477
M300
R411
H744
Products are for research use only. Not for use in diagnostic or therapeutic procedures.