MDA-MB-361 Whole Cell Lysate

MDA-MB-361 Whole Cell Lysate

Product No.: M1006

[product_table name="All Top" skus="M1006"]

- -
- -
Product Type
Cell Line Lysate

- -
- -
Product Size
- -
- -

Details

Cell Line
MDA-MB-361 (Human Breast Adenocarcinoma)
Growth Media
DMEM/Hams F12 & 10% NCS (Newborn Calf Serum)
Preparation
MDA-MB-361 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Host
Human
Concentration
2.0 mg/ml
Formulation
The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.
Expiration Date
One year from date of receipt
Storage
This whole cell lysate is stable for three months at -20°C
Applications and Suggested Working Dilutions
This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.
- -
- -
Products are for research use only. Not for use in diagnostic or therapeutic procedures.