Anti-Mouse γ/δ (TCR) – Purified in vivo GOLD™ Functional Grade

Anti-Mouse γ/δ (TCR) – Purified in vivo GOLD™ Functional Grade

Product No.: T352

[product_table name="All Top" skus="T352"]

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Clone
UC7-13D5
Target
γ/δ T-Cell Receptor
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TCR
Isotype
IgG
Applications
Depletion
,
FC
,
in vivo
,
IP

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Armenian Hamster
Immunogen
Unknown
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Additional Applications Reported In Literature ?
Depletion
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
The anti-mouse γ/δ T-cell receptor antibody reacts with all mouse TCR γ/δ heterodimers.
Antigen Distribution
γδ TCR expression is observed on a subset of cells in the thymus, intestinal epithelium, skin, liver, peripheral lymphoid tissues, and peritoneum. The γδ TCR is involved in the antigen recognition of some bacterial or tumor-associated antigens presented by MHC class I.
Background
The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.

Antigen Details

Ligand/Receptor
Some bacterial or tumor antigens bound MHC class I
Function
Antigen recognition
Research Area
Adaptive Immunity
.
Immunology

References & Citations

1.) Bluestone, J. A et al. (1991) Immunol. Rev. 120:5-33 Journal Link
2.) Hiromatsu K et al. (1992) J. Exp. Med. 175 (1):49-56 Journal Link
3.) van der Heyde HC et al. (1995) J. Immunol. 154 (8): 3985-3990 Journal Link
4.) Pollinger B et al. (2011) J. Immunol. 186 (4):2602-12 Journal Link
Depletion
Flow Cytometry
in vivo Protocol
Immunoprecipitation Protocol
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.