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Anti-Mouse CD3 Purified Low Endotoxin Functional Formulation

(T-cell Receptor Complex, CD3ε)
Monoclonal Antibody


Prod. No.:C2443
Clone:17A2
Isotype:Rat IgG2b κ
Conc.:1.0 mg/ml
Pkg. Size:0.5 mg, 1.0 mg, 5.0 mg, 25 mg, 100 mg
Starting at:$98.00
Availability:In Stock
Storage:Sterile 2-8°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse CD3 (Clone 17A2) recognizes an epitope on Mouse CD3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The 17A2 clone reacts with the ε and γ chains and not δ nor ζ of CD3. T

Antigen Distribution:
The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.

Host Species
Rat
Immunogen
γ/δ TCR-positive T-T hybridoma D1
Formulation
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purity
>95% by SDS-PAGE and HPLC
Endotoxin
<0.5 EU/mg as determined by the LAL method
Storage and Stability
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), FC, IP, In vivo Depletion
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Mysliwietz J et al. (1992) Blood 80: 2661-2667
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