Product Datasheet
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Anti-Mouse CD3ε DyLight® 488

Monoclonal Antibody


Prod. No.:C1729
Clone:500A2
Isotype:Syrian Hamster IgG2a κ
Conc.:0.5 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Syrian Hamster Anti-Mouse CD3ε (Clone 500A2) recognizes an epitope on Mouse CD3ε. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.

Reporter Molecule:
DyLight® 488 conjugated antibodies absorb light maximally around 493 nm (± 4 nm) and fluoresce with a peak around 518 nm. They are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates, but typically give less background. DyLight® 488 conjugated to any antibody is recommended to maximize sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Ms+
Host Species
Syrian Hamster
Immunogen
Purified Recombinant Mouse CD3ε (>98%)
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


References
1. Havran, W. M. et al. (1987) Nature 330:170
2. Allison, J. W. et al. (1988) The T-Cell Receptor 33
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Anti-Mouse CD3ε Purified (Leinco Prod. No.: C207)
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