Product Datasheet
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Anti-Mouse CD3ε DyLight® 550

Monoclonal Antibody


Prod. No.:C1730
Clone:500A2
Isotype:Syrian Hamster IgG2a κ
Conc.:0.5 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Starting at:$85.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Syrian Hamster Anti-Mouse CD3ε (Clone 500A2) recognizes an epitope on Mouse CD3ε. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.

Reporter Molecule:
DyLight® 550 conjugated antibodies absorb light maximally around 562 (± 4 nm) and fluoresce with a peak around 576 nm. They are brighter than TRITC conjugates, and they are equal in brightness to Cy3 and Alexa Fluor 555. All Dylight® 550 antibodies are recommended for maximum sensitivity in all immunofluorescence detection procedures within the orange-red portion of the visible spectrum. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Ms+
Host Species
Syrian Hamster
Immunogen
Purified Recombinant Mouse CD3ε (>98%)
Formulation
This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 550 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Havran, W. M. et al. (1987) Nature 330:170
2. Allison, J. W. et al. (1988) The T-Cell Receptor 33
Related Products
Anti-Mouse CD3ε-Biotin (Leinco Prod. No.: C208)
PN:
Anti-Mouse CD3ε Purified (Leinco Prod. No.: C207)
Anti-Mouse CD3ε Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C219)

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