Product Datasheet
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Anti-Mouse CD3ε DyLight® 680

Monoclonal Antibody


Prod. No.:C1734
Clone:500A2
Isotype:Syrian Hamster IgG2a κ
Conc.:0.5 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C 500A2 Detailed storage instructions below.
Description
Specificity:
Syrian Hamster Anti-Mouse CD3ε (Clone 500A2) recognizes an epitope on Mouse CD3ε. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.

Reporter Molecule:
DyLight® 680 conjugated antibodies absorb light maximally around 682 nm (± 4 nm) and fluoresce maximally around 715 nm. DyLight® 680 produces near-infrared (IR) fluorescence that replaces other near-IR dyes, including Cy5 and Alexa Fluor 680, and is ideal for multi-color applications including fluorescence microscopy, flow cytometry, ELISA. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Ms+
Host Species
Syrian Hamster
Immunogen
Purified Recombinant Mouse CD3ε (>98%)
Formulation
This DyLight® 680 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 680 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Havran, W. M. et al. (1987) Nature 330:170
2. Allison, J. W. et al. (1988) The T-Cell Receptor 33
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Anti-Mouse CD3ε Purified (Leinco Prod. No.: C207)
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