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Anti-Mouse CD3ε (CD3ε) Purified

Monoclonal Antibody


Prod. No.:C207
Clone:500A2
Isotype:Syrian Hamster IgG2a κ
Conc.:0.5 mg/ml
Pkg. Size:50 µg, 500 µg
Starting at:$40.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Syrian Hamster Anti-Mouse CD3ε (Clone 500 A2) recognizes an epitope on Mouse CD3ε. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.

Known Species Cross Reactivity: Ms+
Host Species
Syrian Hamster
Immunogen
Mouse T-cell receptor
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This antibody is stable when stored undiluted at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), FC, IP, FA
Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a working dilution of 1:10 - 1:50.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3ε. A suggested method would be to stain cells expressing CD3ε with ≤0.5 µg per 1 x 106 cells in a total staining volume of 100 µl followed by staining with a suitable secondary antibody.
Functional Assay: Binding of Syrian Hamster Anti-Mouse CD3ε (Clone 500A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation of mouse T cells. However, it is recommended to use Anti-Mouse CD3ε Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C219) for functional assays.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
Havran, W. M. et al. (1987) Nature 330:170
Allison, J. W. et al. (1988) The T-Cell Receptor 33
Related Products
Anti-Mouse CD3ε Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C219)
Anti-Mouse CD3ε-Biotin (Leinco Prod. No.: C208)
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