Product Datasheet
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Anti-Mouse CD3ε (CD3ε) DyLight® 650

(CD3, T3)
Monoclonal Antibody


Prod. No.:C2240
Clone:145-2C11
Isotype:Armenian Hamster IgG
Conc.:0.5 mg/ml
Pkg. Size:50 µg, 100 ug
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Armenian Hamster Anti-Mouse CD3ε (CD3ε) (Clone 145-2C11) recognizes an epitope on Mouse CD3ε. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested.

Background:
CD3ε is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Reporter Molecule:
DyLight® 650 conjugated antibodies absorb light maximally around 652 nm (± 4 nm) and fluoresce maximally around 672 nm. They are brighter than Cy5 and Alexa 647 conjugates plus, they typically give less background than Alexa 647 conjugates. DyLight® 650 antibody conjugates are the best choice for far red-emitting conjugates and for multiple-labeling detection with a confocal microscope. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Ms+
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 650 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC, ICC
Flow Cytometry: This antibody can be used at a concentration of ≤ 0.25 µg per million cells (or per 1.0 x 106 cells in a 100 µl total staining volume).
Immunocytochemistry: Assay Dependent
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Anti-Mouse CD3ε-Biotin (Leinco Prod. No.: C208)
PN:
Anti-Mouse CD3ε Purified (Leinco Prod. No.: C207)
Anti-Mouse CD3ε Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C219)
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