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Anti-Human CD71 (CD71) Allophycocyanin (APC)

(Transferrin Receptor)
Monoclonal Antibody


Prod. No.:C328
Clone:T56/14
Isotype:Mouse IgG1
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CD71 (Clone T56/14) recognizes Human CD71 transferrin receptor (Mr 190 kDa). This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The transferrin receptor is a cell surface glycoprotein related to cell proliferation. Up-regulation of the CD71 antigen is associated with actively dividing cells.1 Transferrin receptors are also present on reticulocytes, but are lost during maturation into erythrocytes.2

Background:
Anti-transferrin receptor may be used to identify activated lymphocytes. The CD71 antibody may be used in studies of erythropoiesis.

Reporter Molecule:
Allophycocyanin (APC) has a molecular weight of 104 kDa and is purified from green algae. APC is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Trowbridge, S. I. et al. (1981) Proc. Nat'l. Acad. Sci. 78:3039
2. Iacopetta, B. J. et al. (1983) J. Histochem. Cytochem. 31:336
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PN:
Anti-Mouse CD71 (Transferrin Receptor)-R-phycoerythrin (Leinco Prod. No.: C278)
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