Anti-Mouse CD86 (Clone GL1) – Purified in vivo GOLD™ Functional Grade

LPS-activated CBA/Ca mouse splenic B cells

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

Clone GL-1 recognizes an epitope on mouse CD86.

CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes.

CD86 is an 80kD Ig superfamily member that is involved in immunoglobulin class-switching and activation of NK cell-mediated cytotoxicity. CD80 is closely related to, and works in tandem with CD86 to prime T- cells. CD86 is expressed earlier in the immune response than CD80. The ligation of CD28 on T cells with CD80 and CD86 on APCs co-stimulates T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD86 can also bind to CTLA-4 to deliver an inhibitory signal to T cells.

CD28, CD152 (CTLA-4)

T cell costimulation, Ig class-switching, NK cell cytotoxicity

1. Hathcock, K.S. et al.. (1993) Science 262(5135:905-7
2.) Gubin, M. et al. (2018) Cell. 175(4):1014–1030.e19 Journal Link