Anti-Mouse CD8a (Ly 2.2) – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse CD8a (Ly 2.2) – Purified in vivo PLATINUM™ Functional Grade

Product No.: C2837

[product_table name="All Top" skus="C2837"]

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Clone
2.43
Target
CD8a
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Ly-2, Ly-35, Ly-B, Lyt-2, Lyt2, Ly 2.2
Isotype
IgG2b
Applications
Depletion
,
FA
,
FC
,
ICC
,
IF Staining
,
IHC FFPE
,
in vivo
,
IP

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Mouse CTL clone L3
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional GOLD™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 2.43 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
Depletion
FA
Additional Reported Applications For Relevant Conjugates ?
ICC
IF Staining
IHC (Paraffin)
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 2.43 recognizes an epitope on mouse CD8a.
Antigen Distribution
CD8a is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic-cells.
Background
CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes.

Antigen Details

NCBI Gene Bank ID

References & Citations

1.) Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668. PubMed
2.) Hawman DW, et al. (2021) Microorganisms 9(2):279 Journal Link
Depletion
FA
Flow Cytometry
ICC
IF Staining
IHC FFPE
in vivo Protocol
Immunoprecipitation Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.