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Anti-Mouse H-2Kb Allophycocyanin (APC)

(MHC Class I)
Monoclonal Antibody


Prod. No.:H1670
Clone:AF6-88.5
Isotype:Mouse IgG2a κ
Conc.:0.1 mg/ml
Pkg. Size:25 µg, 100 µg
Starting at:$80.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Mouse H-2Kb (Clone AF6-88.5.5.3) recognizes an epitope on Mouse H-2Kb. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

Reporter Molecule:
Allophycocyanin (APC) has a molecular weight of 104 kDa and is purified from green algae. APC is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Host Species
Mouse
Immunogen
Mouse C57BL Splenocytes
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: This antibody can be used at a concentration of ≤ 0.25 µg per million cells (or per 1.0 x 106 cells in a 100 µl total staining volume) in 100 µl volume.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
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