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Anti-Mouse H-2Kb DyLight® 488

(MHC Class I)
Monoclonal Antibody


Prod. No.:H1673
Clone:AF6-88.5
Isotype:Mouse IgG2a
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Starting at:$95.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Mouse H-2Kb (Clone AF6-88.5.5.3) recognizes an epitope on Mouse H-2Kb. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

Reporter Molecule:
DyLight® 488 conjugated antibodies absorb light maximally around 493 nm (± 4 nm) and fluoresce with a peak around 518 nm. They are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates, but typically give less background. DyLight® 488 conjugated to any antibody is recommended to maximize sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Host Species
Mouse
Immunogen
Mouse C57BL Splenocytes
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
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